Applying Staining Techniques to View and Identify...

Abstract The main objective of this lab was to identify different bacteria by simple, negative, and gram staining. To view each bacteria cell, the bacteria was transferred aseptically to a slide, and they were then viewed by using oil immersion, by a light microscope. From this lab, it was determined that E. coli and B. megaterium are gram negative and B. subtilis and S. Marcesans are gram positive. Introduction The purpose of this lab was to view the different characteristics of bacteria by applying various staining techniques. It is important to know the make up if a certain bacteria so an antibiotic may be engineered to destroy the bacteria. From the gram stain, it was possible to determine which bacteria was gram positive†¦show more content†¦Distilled water Three different staining procedures were then used for all four types of bacteria. The directions for each staining process can be found on pages 18-19 of the lab manual. For simple stain, bacteria was removed with a sterile inoculating loop and placed on a glass slide. Methylene blue was then applied until the slide was covered. Distilled water was then poured on the slide until the methylene blue was removed. The slide was allowed to dry. Next, oil was placed on the slide so that the oil objective lens on the light microscope was employed. The bacterium was viewed and a sketch was made. For the negative stain, a loopful of bacteria was placed on a slide. A drop of Nigrosin solution was placed next to the bacteria, and the blood smearing technique learned in the first lab was applied to cover the slide with nigrosin solution. A sketch was then made. Gram staining was more complicated. First, two separate types of bacteria were used. One type was placed on one slide, and the other bacteria were placed on the opposite side. Both bacteria were also placed in the middle of the slide so they could be viewed together. Crystal violet, iodine and safranin were all placed on the slide and washed off with distilled water. Once dried, oil was applied to the slide, and it was ready to be viewed. Again a sketch was made. Results The sketches were drawn on a separate piece of paper and then revised. AShow MoreRelatedIdentify The Identities Of Two Unknown Bacteria A Mixed Broth Culture By Using Several Separation Methods Essay1441 Words   |  6 PagesAbstract: The purpose of this project was to identify the identities of two unknown bacteria in a mixed broth culture by using several separation methods. To separate the organisms, a four-way streak plate technique was used to isolate the two unknown bacteria into separate visible colonies. Then after each colony were clearly isolated; the two unknowns were processed through Gram staining test to determine the Gram stain and morphology. After Gram staining, a carbohydrate test was performed on eachRead MoreLab Report On Microbial Unknowns Essay1793 Words   |  8 PagesUnknowns Objective: In the past couple of weeks, our class received a single broth that contained two species of bacterium. One bacterium was gram positive and one bacterium was gram negative. We were required to use our techniques and knowledge we learned in class to separate and identify the two species we were given in our broths. Methodology: Day One: On the first day of our Biochemical Unknown Lab we were given a tube that contained broth media which held two different types of bacterium. OnceRead MoreCommon Laboratory Operations5997 Words   |  24 Pagesinvented the mechanical calculator, the syringe and created hydraulic press   Rene Descartes(1596-1650) A French philosopher and mathematician. He was the first major philosopher in the modern era to make a serious effort to defeat skepticism. His views about knowledge and certainty and those on the relationship between mind and body have been very influential over the last three centuries. I am thinking there fore I exist (Latin -Cogito ergo sum- from the discourse on method). He revelolutionizedRead MoreCleaning Support Portfolio - Hotel Management12907 Words   |  52 PagesCLEANING SUPPORT TECHNIQUES Team 9.2.3 EHLs Honour Code: As a student at the Ecole Hà ´telià ¨re de Lausanne, I uphold and defend academic integrity, academic rigor and academic liberty as core values of higher learning. I attest, on my word of honor, that work submitted in my name is my own work, and that any ideas or materials used in support of this work which are not originally my own are cited and referenced accordingly BINGGELI Vanessa DANG Yung GOUVEA Priscila KRAMER Toby ORLANDO Giulia SCHNYDER